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1 May 2006 An In Vivo Study of Free Radicals Generated in Murine Skin by Protoporphyrin IX and Visible Light
Kozo Nakai, Ann G. Motten, Colin F. Chignell
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Abstract

Lipids extracted from the skin of C57BL/6J mice injected subcutaneously with α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN) and exposed to topical protoporphyrin IX (PPIX) and visible light had significantly higher levels of POBN spin adducts compared with dark PPIX exposed or vehicle-treated controls. Computer analysis of the POBN adduct electron paramagnetic (spin) resonance (EPR) spectra indicated that two radical species were present in each extract, one of which was a lipid-derived carbon-centered adduct (1, aN = 14.8 G and aH = 2.6 G), whereas the other (2, aN = 13.8 G and aH = 1.8 G) was probably oxygen centered. Adduct 2 was present in greater proportion in lipids extracted from PPIX/light-exposed mice compared with dark or vehicle-treated controls. These findings suggest that PPIX/light generates free radicals in mouse skin, thus providing a radical mechanism for PPIX-induced photosensitivity. Our approach may be useful for the detection of free radicals generated by other skin photosensitizers and may also provide a means for testing putative skin-protecting agents.

Kozo Nakai, Ann G. Motten, and Colin F. Chignell "An In Vivo Study of Free Radicals Generated in Murine Skin by Protoporphyrin IX and Visible Light," Photochemistry and Photobiology 82(3), 738-740, (1 May 2006). https://doi.org/10.1562/2006-01-30-RA-787
Received: 30 January 2006; Accepted: 1 March 2006; Published: 1 May 2006
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