Collections from small permanent or ephemeral surface waterbodies, some of them brackish, in the Florida Keys yielded 2 species of calanoid, 17 species of cyclopoid, and 11 species and subspecies of harpacticoid copepod crustaceans. Most of the species are either cosmopolitan or widely distributed in the Neotropics. Even so, these collections have established new records for the U.S.A. for Mastigodiaptomus nesus, Halicyclops bowmani, Paracyclops bromeliacola, Nitokra lacustris sinoi, and Schizopera tobae cubana, and new records for the state of Florida for Apocyclops dimorphus, Diacyclops bernardi, Cletocamptus fourchensis, Nitocrella aestuarina, Pseudectinosoma cf. minor, and Tisbella pulchella. Already known from two locations in central Florida, Bryocyclops muscicola was found in a bromeliad on Duck Key. Another species found in a bromeliad, Paracyclops bromeliacola, was previously known only from Brazil; like B. muscicola, this species may have been introduced into Florida by human agency.