Specimens comprising the species Tripudia quadrifera (Zeller) and Tripudia grapholithoides (Möschler) were discovered to contain six new species: Tripudia rectangula, n. sp., from the eastern United States; Tripudia paraplesia, n. sp., from eastern Mexico; Tripudia flavibrunnea, n. sp., from Mexico, Guatemala, and the Dominican Republic; Tripudia lamina, n. sp., from eastern Mexico, Central America, the Caribbean, Venezuela, and Ecuador; Tripudia furcula, n. sp., from El Salvador and Honduras; and Tripudia fabrilium, n. sp., from Pernambuco, Brazil. Adults and male and female genitalia are illustrated. Known collection sites of all species are mapped. Keys to the male and female genitalia are provided, as this is the only reliable way to separate these species.