The genus Ecdytolopha Zeller, 1875 includes 14 described species whose geographic distributions encompass most of temperate North America and a large part of the northern Neotropics. Two new species are described: Ecdytolopha nigrita Brown, new species, from southeastern Arizona, U.S.A.; and Ecdytolopha meridana Brown, new species, from Merida, Venezuela. A neighbor-joining tree based on COI sequences of Ecdytolopha insiticiana Zeller, E. mana (Kearfott), E. occidentana Adamski and Brown, E. nigrita, and E. fabivora (Meyrick) is presented. Although percent divergence within species is typical for Lepidoptera, divergences between species of Ecdytolopha are fairly large, with the most similar pair (i.e., E. insiticiana and E. mana) 9.1% different.