Two new species of Neotrichia (Hydroptilidae) from Mexico are described, Neotrichia barbai new species and Neotrichia marinoi new species. The male genitalia of both species are illustrated. Neotrichia barbai new species can be recognized by the inferior appendages with apices curved outward, posterior margin of segment IX with a narrow lateral process, and phallus with long, straight paramere and stout, hook-like sclerite. Neotrichia marinoi new species can be recognized by the deltoid shape of the bracteole in ventral view, phallus with long, straight paramere and the long, narrow, ventroanteriorly produced subgenital plate. The two new species were phylogenetically placed within the N. vibrans species group, based on the subgenital plate narrow, rod-like and with a shallow incision. A list of species in the genus Neotrichia is provided in alphabetical order, which shows their distribution in Mexico and includes seven new state records and the two new species.