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1 June 2002 Radiation-Induced Changes in Permeability in Unilamellar Phospholipid Liposomes
Dipti Marathe, K. P. Mishra
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Marathe, D. L. and Mishra, K. P. Radiation-Induced Changes in Permeability in Unilamellar Phospholipid Liposomes. Radiat. Res. 157, 685–692 (2002).

Gamma-radiation-induced oxidative damage in unilamellar dipalmitoylphosphatidylcholine liposomes was investigated using a fluorescence technique. Liposomal changes in permeability induced by gamma radiation were monitored by measuring the leakage of pre-encapsulated 6-carboxyfluorescein, and alterations in lipid bilayer fluidity were determined by 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization. The changes in permeability and fluidity in the bilayer were found to be dependent on the radiation dose in a biphasic fashion. The results are interpreted in terms of lipid bilayer fluidization after exposure to doses up to 1 kGy, but rigidization of the bilayer at higher doses. These results indicate a relationship between alterations in permeability and fluidity in the lipid bilayer after irradiation. The vesicles were protected significantly against radiation-induced oxidative damage in the presence of α-tocopherol and ascorbic acid. Radiation-induced changes in the permeability of the liposomes after exposure to γ radiation and their modification by antioxidants indicate the involvement of a free radical mechanism in the production of damage, which may offer new insights in to the modification of cellular radiosensitivity by modulation of membrane damage.

Dipti Marathe and K. P. Mishra "Radiation-Induced Changes in Permeability in Unilamellar Phospholipid Liposomes," Radiation Research 157(6), 685-692, (1 June 2002).[0685:RICIPI]2.0.CO;2
Received: 9 July 2001; Accepted: 1 January 2002; Published: 1 June 2002

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