Translator Disclaimer
1 August 2003 Radiosensitization by Inhibition of IκB-α Phosphorylation in Human Glioma Cells
Gui-Rong Ding, Naoko Honda, Takehisa Nakahara, Furong Tian, Masami Yoshida, Hideki Hirose, Junji Miyakoshi
Author Affiliations +
Abstract

Ding, G-R., Honda, N., Nakahara, T., Tian, F., Yoshida, M., Hirose, H. and Miyakoshi, J. Radiosensitization by Inhibition of IκB-α Phosphorylation in Human Glioma Cells. Radiat. Res. 160, 232–237 (2003).

To assess the role of nuclear factor κB (NFKB) in cellular radiosensitivity, three different IκB-α (also known as NFKBIA) expression plasmids, i.e., S-IκB (mutations at 32, 36Ser), Y-IκB (a mutation at 42Tyr), and SY-IκB, were constructed and introduced into human brain tumor M054 cells. The clones were named as M054-S8, M054-Y2 and M054-SY4, respectively. Compared to the parental cell line, M054-S8 and M054-Y2 cells were more sensitive to X rays while M054-SY4 cells exhibited the greatest sensitivity. After treatment with N-acetyl-Leu-Leu-norleucinal, a proteasome inhibitor, the X-ray sensitivity of M054-S8 and M054-SY4 cells did not change, while that of M054-Y2 cells and the parental cells was enhanced. An increase in X-ray sensitivity accompanied by a decrease in translocation of NFKB to the nucleus in parental cells was observed after treatment with pervanadate, an inhibitor of tyrosine phosphatase, as well as in M054-S8 and M054-SY4 cells. Repair of potentially lethal damage (PLD) was observed in the parental cells but not in the clones. Four hours after irradiation (8 Gy), the expression of TP53 and phospho-p53 (15Ser) was induced in the parental cells but not in M054-S8, M054-Y2 or M054-SY4 cells. Our data suggest that inhibition of IκB-α phosphorylation at serine or tyrosine acts independently in sensitizing cells to X rays. NFKB may play a role in determining radiosensitivity and PLD repair in malignant glioma cells; TP53 may also be involved.

Gui-Rong Ding, Naoko Honda, Takehisa Nakahara, Furong Tian, Masami Yoshida, Hideki Hirose, and Junji Miyakoshi "Radiosensitization by Inhibition of IκB-α Phosphorylation in Human Glioma Cells," Radiation Research 160(2), 232-237, (1 August 2003). https://doi.org/10.1667/RR3018
Received: 28 May 2002; Accepted: 1 March 2003; Published: 1 August 2003
JOURNAL ARTICLE
6 PAGES


Share
SHARE
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top