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1 April 2004 Ion Desorption from DNA Components Irradiated with 0.5 keV Ultrasoft X-Ray Photons
Kentaro Fujii, Ken Akamatsu, Akinari Yokoya
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Abstract

Fujii, K., Akamatsu, K. and Yokoya, A. Ion Desorption from DNA Components by Ultrasoft X-Ray Photons. Radiat. Res. 161, 435–441 (2004).

Positive ion desorption from thin films of DNA components, 2-deoxy-d-ribose, thymine, thymidine (dThd), and thymidine 5′-monophosphate (dTMP) was investigated in the oxygen K- shell edge excitation region using synchrotron ultrasoft X rays (538 eV). A large number of molecular fragments, H , CHx , C2Hx , CO , CHxO , C3Hx , C2HxO and C3HxO (x = 1, 2 and 3), were observed as desorbed ions from 2-deoxy-d-ribose. Some of these ions are related to simultaneous bond scission at particular C–C and C–O (or C–C) bonds in the furanose ring structure in the 2-deoxy-d-ribose molecule, indicating that the impact of photons on the oxygen atom and the impact of ejected secondary electrons (e.g. Auger electrons) cause an intense destruction of the furanose ring structure. In thymine thin films, H , CHx , CO , CHxO , C2HxN and CHxNO (x = 1, 2 and 3) fragments were observed. The yields of these ions were smaller than the yields from 2-deoxy-d-ribose. The desorption of CH3 from thymine might induce a molecular conversion from thymine to uracil. The mass patterns of dThd and dTMP, and especially that of dTMP, were similar to that of 2-deoxy-d-ribose, indicating that a number of ions were generated at the sugar site, even in the nucleotide molecule. It is therefore predicted that the sugar moiety is more fragile than the thymine base.

Kentaro Fujii, Ken Akamatsu, and Akinari Yokoya "Ion Desorption from DNA Components Irradiated with 0.5 keV Ultrasoft X-Ray Photons," Radiation Research 161(4), 435-441, (1 April 2004). https://doi.org/10.1667/RR3131
Received: 3 February 2003; Accepted: 1 September 2003; Published: 1 April 2004
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