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1 April 2004 A Genome Scanning Approach to Assess the Genetic Effects of Radiation in Mice and Humans
Jun-ichi Asakawa, Rork Kuick, Mieko Kodaira, Nori Nakamura, Hiroaki Katayama, Donald Pierce, Sachiyo Funamoto, Dale Preston, Chiyoko Satoh, James V. Neel, Samir Hanash
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Abstract

Asakawa, J., Kuick, R., Kodaira, M., Nakamura, N., Katayama, H., Pierce, D., Funamoto, S., Preston, D., Satoh, C., Neel, J. V. and Hanash, S. A Genome Scanning Approach to Assess the Genetic Effects of Radiation in Mice and Humans. Radiat. Res. 161, 380–390 (2004).

We used Restriction Landmark Genome Scanning (RLGS) to assess, on a genome-wide basis, the mutation induction rate in mouse germ cells after radiation exposure. Analyses of 1,115 autosomal NotI DNA fragments per mouse for reduced spot intensity, indicative of loss of one copy, in 506 progeny derived from X-irradiated spermatogonia (190, 237 and 79 mice in 0-, 3-, and 5-Gy groups, respectively), permitted us to identify 16 mutations affecting 23 fragments in 20 mice. The 16 mutations were composed of eight small changes (1–9 bp) at microsatellite sequences, five large deletions (more than 25 kb), and three insertions of SINE B2 or LINE1 transposable elements. The maximum induction rate of deletion mutations was estimated as (0.17 ± 0.09) × 10−5/locus Gy–1. The estimate is considerably lower than 1 × 10−5/locus Gy–1, the mean induction rate of deletion mutations at Russell's 7 loci, which assumed that deletion mutations comprise 50% of all mutations. We interpret the results as indicating that the mean induction rate of mutations in the whole genome may be substantially lower than that at the 7 loci. We also demonstrate the applicability of RLGS for detection of human mutations, which allows direct comparisons between the two species.

Jun-ichi Asakawa, Rork Kuick, Mieko Kodaira, Nori Nakamura, Hiroaki Katayama, Donald Pierce, Sachiyo Funamoto, Dale Preston, Chiyoko Satoh, James V. Neel, and Samir Hanash "A Genome Scanning Approach to Assess the Genetic Effects of Radiation in Mice and Humans," Radiation Research 161(4), 380-390, (1 April 2004). https://doi.org/10.1667/RR3146
Received: 29 September 2003; Accepted: 1 November 2003; Published: 1 April 2004
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