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1 May 2004 The Role of Bcl-XS in Radiation Sensitivity
M. A. Morgan, J. N. Poynter, J. Maybaum, T. S. Lawrence
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Morgan, M. A., Poynter, J. N., Maybaum, J. and Lawrence, T. S. The Role of Bcl-XS in Radiation Sensitivity. Radiat. Res. 161, 535–539 (2004).

Bcl-XS is a pro-apoptosis member of the Bcl2 family that has been shown to induce cell death and enhance chemosensitivity. We have investigated the effect of Bcl-XS overexpression on radiation sensitivity. Using a tetracycline-repressible system, we found that removal of tetracycline for 16 h induced Bcl-XS and reduced the surviving fraction of NIH 3T3 cells to 25%. However, radiation sensitivity was not significantly affected by Bcl-XS expression; the mean inactivation doses for Bcl-XS repressed and Bcl-XS induced cells were 2.7 ± 0.3 and 2.3 ± 0.1 Gy, respectively. We conclude that Bcl-XS induces cell death without affecting radiation sensitivity. These results suggest that mitochondrial pathways to apoptosis may not have a significant role in survival after irradiation.

M. A. Morgan, J. N. Poynter, J. Maybaum, and T. S. Lawrence "The Role of Bcl-XS in Radiation Sensitivity," Radiation Research 161(5), 535-539, (1 May 2004).
Received: 31 July 2003; Accepted: 1 January 2004; Published: 1 May 2004

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