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1 January 2005 Bound PCNA in Nuclei of Primary Rat Tracheal Epithelial Cells after Exposure to Very Low Doses of Plutonium-238 α Particles
M. A. Hill, J. R. Ford, P. Clapham, S. J. Marsden, D. L. Stevens, K. M S. Townsend, D. T. Goodhead
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Abstract

Hill, M. A., Ford, J. R., Clapham, P., Marsden, S. J., Stevens, D. L., Townsend, K. M. S. and Goodhead, D. T. Bound PCNA in Nuclei of Primary Rat Tracheal Epithelial Cells after Exposure to Very Low Doses of Plutonium-238 α Particles. Radiat. Res. 163, 36–44 (2005).

Bystander effects from ionizing radiation have been detailed for a number of cell systems and a number of end points. We wished to use a cell culture/ex vivo rat model of respiratory tissue to determine whether a bystander effect detected in culture could also be shown in a tissue. Examination by immunofluorescence techniques of tracheal cell cultures after exposure to very low doses of α particles revealed a large proportion of cells with proliferating cell nuclear antigen (PCNA) bound in their nuclei. PCNA was selected as an end point because it is involved in both DNA repair and the changes in cell cycle that are typical of many reported bystander effects. Maximum response can be detected in up to 28% of the cells in sub-confluent cultures with a dose of only 2 mGy. At this dose less than 2% of the cell nuclei have experienced a particle traversal and less than 6% of the cells have experienced an α-particle traversal through either their nucleus or some part of their cytoplasm. The hypothesis that this bystander response in nontargeted cells is mediated through secreted factor(s) is presented, and supporting evidence was found using partial irradiation and co-culture experiments. Examination of the effect with excised pieces of trachea demonstrated a response similar to that seen in culture.

M. A. Hill, J. R. Ford, P. Clapham, S. J. Marsden, D. L. Stevens, K. M S. Townsend, and D. T. Goodhead "Bound PCNA in Nuclei of Primary Rat Tracheal Epithelial Cells after Exposure to Very Low Doses of Plutonium-238 α Particles," Radiation Research 163(1), 36-44, (1 January 2005). https://doi.org/10.1667/RR3282
Received: 29 December 2003; Accepted: 1 August 2004; Published: 1 January 2005
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