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1 June 2005 Radiation-Induced Hydroxyl Addition to Purine Molecules: EPR and ENDOR Study of Hypoxanthine Hydrochloride Monohydrate Single Crystals
Sibel Tokdemir, William H. Nelson
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Abstract

Tokdemir, S. and Nelson, W. H. Radiation-Induced Hydroxyl Addition to Purine Molecules: EPR and ENDOR Study of Hypoxanthine Hydrochloride Monohydrate Single Crystals. Radiat. Res. 163, 663–672 (2005).

Three radical species were detected in an EPR/ENDOR study of X-irradiated hypoxanthine·HCl·H2O single crystals at room temperature: RI was identified as the product of net H addition to C8, RII was identified as the product of net H addition to C2, and RIII was identified as the product of OH addition to C8. The observed set of radicals was the same for room-temperature irradiation as for irradiation at 10 K followed by warming the crystals to room temperature; however, the C2 H-addition and C8 OH-addition radicals were not detectable after storage of the crystals for about 2 months at room temperature. Use of selectively deuterated crystals permitted unique assignment of the observed hyperfine couplings, and results of density functional theory calculations on each of the radical structures were consistent with the experimental results. Comparison of these experimental results with others from previous crystal-based systems and model system computations provides insight into the mechanisms by which the biologically important purine C8 hydroxyl addition products are formed. The evidence from solid systems supports the mechanism of net water addition to one-electron oxidized purine bases and demonstrates the importance of a facial approach between the reactants.

Sibel Tokdemir and William H. Nelson "Radiation-Induced Hydroxyl Addition to Purine Molecules: EPR and ENDOR Study of Hypoxanthine Hydrochloride Monohydrate Single Crystals," Radiation Research 163(6), 663-672, (1 June 2005). https://doi.org/10.1667/RR3375
Received: 20 December 2004; Accepted: 1 February 2005; Published: 1 June 2005
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