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1 January 2007 TP53 and TP53-Related Genes Associated with Protection from Apoptosis in the Radioadaptive Response
Ryuji Okazaki, Akira Ootsuyama, Toshiyuki Norimura
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Abstract

Okazaki, R., Ootsuyama, A. and Norimura, T. TP53 and TP53-Related Genes Associated with Protection from Apoptosis in the Radioadaptive Response. Radiat. Res. 167, 51–57 (2007).

We investigated the effect of administering priming low-dose radiation prior to high-dose radiation on the level of apoptosis and on the expression of TP53 and TP53-related genes in mouse splenocytes. The percentage of apoptotic cells was significantly lower in TP53 / mice receiving priming radiation 2 to 168 h before the high-dose irradiation, compared to TP53 / mice exposed to 2 Gy alone. In contrast, TP53 /− mice exhibited a reduced level of apoptosis only when priming was performed for 2 or 4 h prior to the high-dose irradiation. In TP53 / mice, primed mice had higher TP53 expression than mice exposed to 2 Gy. Phospho-TP53 (ser15/18) expression was the highest in mice exposed to 2 Gy and intermediate in primed mice. Expression of p21 (CDKN1A) was higher in primed mice compared with mice exposed to 2 Gy. MDM2 expression remained at a high level in all mice receiving 2 Gy. Elevated phospho-ATM expression was observed only in mice exposed to 2 Gy. We conclude that TP53 plays a critical role in the radioadaptive response and that TP53 and TP53-related genes might protect cells from apoptosis through activation of the intracellular repair system.

Ryuji Okazaki, Akira Ootsuyama, and Toshiyuki Norimura "TP53 and TP53-Related Genes Associated with Protection from Apoptosis in the Radioadaptive Response," Radiation Research 167(1), 51-57, (1 January 2007). https://doi.org/10.1667/RR0623.1
Received: 14 March 2006; Accepted: 1 September 2006; Published: 1 January 2007
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