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1 February 2007 Novel Radiation Response Genes Identified in Gene-Trapped MCF10A Mammary Epithelial Cells
Jennifer Malone, Robert Ullrich
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Abstract

Malone, J. and Ullrich, R. Novel Radiation Response Genes Identified in Gene-Trapped MCF10A Mammary Epithelial Cells. Radiat. Res. 167, 176–184 (2007).

We have used a gene-trapping strategy to screen human mammary epithelial cells for radiation response genes. Relative mRNA expression levels of five candidate genes in MCF10A cells were analyzed, both with and without exposure to radiation. In all five cases, the trapped genes were significantly down-regulated after radiation treatment. Sequence analysis of the fusion transcripts identified the trapped genes: (1) the human androgen receptor, (2) the uncharacterized DREV1 gene, which has known homology to DNA methyltransferases, (3) the human creatine kinase gene, (4) the human eukaryotic translation elongation factor 1 beta 2, and (5) the human ribosomal protein L27. All five genes were down-regulated significantly after treatment with varying doses of ionizing radiation (0.10 to 4.0 Gy) and at varying times (2–30 h after treatment). The genes were also analyzed in human fibroblast and lymphoblastoid cell lines to determine whether the radiation response being observed was cell-type specific. The results verified that the observed radiation response was not a cell-type-specific phenomenon, suggesting that the genes play essential roles in the radiation damage control pathways. This study demonstrates the potential of the gene-trap approach for the identification and functional analysis of novel radiation response genes.

Jennifer Malone and Robert Ullrich "Novel Radiation Response Genes Identified in Gene-Trapped MCF10A Mammary Epithelial Cells," Radiation Research 167(2), 176-184, (1 February 2007). https://doi.org/10.1667/RR0656.1
Received: 13 April 2006; Accepted: 1 August 2006; Published: 1 February 2007
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