Translator Disclaimer
1 December 2007 UVB-Radiation-Induced Apoptosis in Jurkat Cells: A Coordinated Fourier Transform Infrared Spectroscopy-Flow Cytometry Study
Deleana Pozzi, Paola Grimaldi, Silvia Gaudenzi, Lucia Di Giambattista, Ida Silvestri, Stefania Morrone, Agostina Congiu Castellano
Author Affiliations +
Abstract

Pozzi, D., Grimaldi, P., Gaudenzi, S., Di Giambattista, L., Silvestri, I., Morrone, S. and Congiu Castellano, A. UVB-Radiation-Induced Apoptosis in Jurkat Cells: A Coordinated Fourier Transform Infrared Spectroscopy-Flow Cytometry Study. Radiat. Res. 168, 698–705 (2007).

We studied the induction of apoptosis in Jurkat cells by UVB radiation (wavelength 290–320 nm) at a dose of 310 mJ/ cm2. We combined Fourier transform infrared (FTIR) spectroscopy with flow cytometry to determine whether the combination of both techniques could provide new and improved information about cell modifications. To do this, we looked for correspondences and correlations between spectroscopy and flow cytometry data and found three highly probable spectroscopic markers of apoptosis. The behavior of the wave number shift of both the Amide I β-sheet component and the area of the 1083 cm−1 band reproduced, with a high correlation, the behavior of the early apoptotic cell population, while the behavior of the Amide I area showed a high correlation with the early plus late apoptotic cell population.

Deleana Pozzi, Paola Grimaldi, Silvia Gaudenzi, Lucia Di Giambattista, Ida Silvestri, Stefania Morrone, and Agostina Congiu Castellano "UVB-Radiation-Induced Apoptosis in Jurkat Cells: A Coordinated Fourier Transform Infrared Spectroscopy-Flow Cytometry Study," Radiation Research 168(6), 698-705, (1 December 2007). https://doi.org/10.1667/RR0991.1
Received: 6 February 2007; Accepted: 1 July 2007; Published: 1 December 2007
JOURNAL ARTICLE
8 PAGES


Share
SHARE
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top