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1 November 2008 Differential Gene Signatures in Rat Mammary Tumors Induced by DMBA and Those Induced by Fractionated γ Radiation
Hae-June Lee, Yoon-Jin Lee, Chang-Mo Kang, Sangwoo Bae, Dooil Jeoung, Ja-June Jang, Seung-Sook Lee, Chul-Koo Cho, Yun-Sil Lee
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Abstract

Lee, H-J., Lee, Y-J., Kang, C-M., Bae, S., Jeoung, D., Jang, J-J., Lee, S-S., Cho, C-K. and Lee, Y-S. Differential Gene Signatures in Rat Mammary Tumors Induced by DMBA and Those Induced by Fractionated γ Radiation. Radiat. Res. 170, 579–590 (2008).

The aim of this work was to identify specific genes involved in rat mammary tumors induced by dimethylbenz(a)anthracene (DMBA) or radiation. More TUNEL- and PCNA-positive cells were present in mammary tumors induced by radiation than in tumors induced by DMBA, whereas DNA damage responses like p53 accumulation and histone H2AX phosphorylation were higher in DMBA-induced tumors, even though the pathology was similar in both types of tumors. cDNA microarray and real-time RT-PCR analysis of radiation- or DMBA-induced tumor tissues, revealed that stanniocalcin 2 (Stc2), interferon regulatory factor 1 (Irf1), interleukin 18 binding protein (Il18bp), and chloride channel calcium activated 3 (Clca3) were expressed in both, and that arachidonate 5-lipoxygenase activating protein 1 (Alox5ap) and cathepsin S (Ctss) were expressed only in radiation-induced tumors. No DMBA-specific gene signatures were found. Soft agar growth assays were carried out to identify the carcinogenic features of these specific genes. Cells stably transfected with Alox5ap, Ctss, Stc2, Irf1, Il18bp and Clca3 showed morphological changes compared to controls. These findings indicate different gene alterations in carcinogen- or radiation-induced mammary tumors with similar pathological stages.

Hae-June Lee, Yoon-Jin Lee, Chang-Mo Kang, Sangwoo Bae, Dooil Jeoung, Ja-June Jang, Seung-Sook Lee, Chul-Koo Cho, and Yun-Sil Lee "Differential Gene Signatures in Rat Mammary Tumors Induced by DMBA and Those Induced by Fractionated γ Radiation," Radiation Research 170(5), 579-590, (1 November 2008). https://doi.org/10.1667/RR1106.1
Received: 29 May 2007; Accepted: 1 July 2008; Published: 1 November 2008
JOURNAL ARTICLE
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