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1 March 2009 Selective Radiation-Induced Generation of 2-Deoxyribonolactone Lesions in DNA Mediated by Aromatic Iodonium Derivatives
Marina Roginskaya, Yuriy Razskazovskiy
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Abstract

Roginskaya, M. and Razskazovskiy, Y. Selective Radiation-Induced Generation of 2-Deoxyribonolactone Lesions in DNA Mediated by Aromatic Iodonium Derivatives. Radiat. Res. 171, 342–348 (2009).

2-Deoxyribonolactone lesions were identified as major products of radiation damage to DNA mediated by o,o′-diphenyleneiodonium cations in a hydroxyl radical-scavenging environment. The highest selectivity toward deoxyribonolactone formation (up to 86% of all sugar-phosphate damages) and the overall reaction efficiency (up to 40% of all radiation-generated intermediates converted into products) was displayed by derivatives with positively charged (2-aminoethylthio)acetylamino and (2-aminoethylamino)acetylamino side chains. The reaction can be useful for random single-step incorporation of deoxyribonolactone lesions into single- and double-stranded oligonucleotides and highly polymerized DNA directly in commonly used buffers (PBS, phosphate, Tris-HCl, etc.) at room temperature. In combination with HPLC separation, this technique can serve as a source of short (<6 mer) sequences containing deoxyribonolactone lesions at known positions.

Marina Roginskaya and Yuriy Razskazovskiy "Selective Radiation-Induced Generation of 2-Deoxyribonolactone Lesions in DNA Mediated by Aromatic Iodonium Derivatives," Radiation Research 171(3), 342-348, (1 March 2009). https://doi.org/10.1667/RR1574.1
Received: 22 August 2008; Accepted: 1 October 2008; Published: 1 March 2009
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