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1 April 2009 Ionizing Radiation Induces Microhomology-Mediated End Joining in trans in Yeast and Mammalian Cells
Zorica Scuric, Cecilia Y. Chan, Kurt Hafer, Robert H. Schiestl
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Abstract

Scuric, Z., Chan, C. Y., Hafer, K. and Schiestl, R. H. Ionizing Radiation Induces Microhomology-Mediated End Joining in trans in Yeast and Mammalian Cells. Radiat. Res. 171, 454–463 (2009).

DNA double-strand breaks repaired through nonhomologous end joining require no extended sequence homology as a template for the repair. A subset of end-joining events, termed microhomology-mediated end joining, occur between a few base pairs of homology, and such pathways have been implicated in different human cancers and genetic diseases. Here we investigated the effect of exposure of yeast and mammalian cells to ionizing radiation on the frequency and mechanism of rejoining of transfected unirradiated linear plasmid DNA. Cells were exposed to γ radiation prior to plasmid transfection; subsequently the rejoined plasmids were recovered and the junction sequences were analyzed. In irradiated yeast cells, 68% of recovered plasmids contained microhomologies, compared to only 30% from unirradiated cells. Among them 57% of events used ≥4 bp of microhomology compared to only 11% from unirradiated cells. In irradiated mammalian cells, 54% of plasmids used ≥4 bp of microhomology compared to none from unirradiated cells. We conclude that exposure of yeast and mammalian cells to radiation prior to plasmid transfection enhances the frequency of microhomology-mediated end-joining events in trans. If such events occur within genomic locations, they may be involved in the generation of large deletions and other chromosomal aberrations that occur in cancer cells.

Zorica Scuric, Cecilia Y. Chan, Kurt Hafer, and Robert H. Schiestl "Ionizing Radiation Induces Microhomology-Mediated End Joining in trans in Yeast and Mammalian Cells," Radiation Research 171(4), 454-463, (1 April 2009). https://doi.org/10.1667/RR1329.1
Received: 2 January 2008; Accepted: 1 November 2008; Published: 1 April 2009
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