Translator Disclaimer
1 August 2009 Up-regulation of 8-oxo-dGTPase Activity of MTH1 Protein in the Brain, Testes and Kidneys of Mice Exposed to 137Cs γ Radiation
Karol Bialkowski, Anna Szpila, Kazimierz S. Kasprzak
Author Affiliations +

Mammalian MTH1 protein is an antimutagenic (2′-deoxy)ribonucleoside 5′-triphosphate pyrophosphohydrolase that prevents the incorporation of oxidatively modified nucleotides into nucleic acids. It decomposes most specifically the miscoding products of oxidative damage to purine nucleic acid precursors (e.g. 8-oxo-dGTP, 2-oxo-dATP, 2-oxo-ATP, 8-oxo-GTP) that may cause point mutations or transcription errors when incorporated into DNA and RNA, respectively. The increased expression of MTH1 mRNA and MTH1 protein was previously proposed as a molecular marker of oxidative stress. Therefore, we hypothesized that increased 8-oxo-dGTPase activity of MTH1 protein in mouse organs could serve as a dose-dependent marker of exposure to ionizing radiation, which is known to induce oxidative stress. To test our hypothesis, we measured 8-oxo-dGTPase activity in six organs of male BL6 mice after exposure to 0, 10, 25 and 50 cGy and 1 Gy of 137Cs γ radiation given as a single whole-body dose (1 Gy/min). The mice were killed 4, 8 and 24 h after irradiation. A statistically significant induction of 8-oxo-dGTPase was found in brains, testes and kidneys but not in lungs, hearts or livers. Brains, which demonstrated the highest (4.3-fold) increase of 8-oxo-dGTPase activity, were shown to express ∼50% higher levels of MTH1 protein. However, due to the lack of a simple positive correlation between the dose and the observed 8-oxo-dGTPase activity in brain, testes and kidneys, we conclude that measurements of 8-oxo-dGTPase activity in these organs may serve as a rough indicator rather than a quantifiable marker of radiation-induced oxidative stress.

Karol Bialkowski, Anna Szpila, and Kazimierz S. Kasprzak "Up-regulation of 8-oxo-dGTPase Activity of MTH1 Protein in the Brain, Testes and Kidneys of Mice Exposed to 137Cs γ Radiation," Radiation Research 172(2), 187-197, (1 August 2009).
Received: 16 October 2008; Accepted: 1 March 2009; Published: 1 August 2009

Get copyright permission
Back to Top