The usefulness of laser plasma X-ray pulses for medical and radiation biological studies was investigated, and the effects of laser plasma X rays were compared with those of conventional sources such as a linear accelerator. A cell irradiation system was developed that used copper-Kα (8 keV) lines from an ultrashort high-intensity laser to produce plasma. The absorbed dose of the 8 keV laser plasma X-ray pulse was estimated accurately with Gafchromic® EBT film. When the cells were irradiated with approximately 2 Gy of laser plasma X rays, the circular regions on γ-H2AX-positive cells could be clearly identified. Moreover, the numbers of γ-H2AX and phosphorylated ataxia telangiectasia mutated (ATM) foci induced by 8 keV laser plasma X rays were comparable to those induced by 4 MV X rays. These results indicate that the laser plasma X ray source may be useful for radiation biology studies.
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18 August 2010
γ-H2AX and Phosphorylated ATM Focus Formation in Cancer Cells after Laser Plasma X Irradiation
Katsutoshi Sato,
Masaharu Nishikino,
Yasuaki Okano,
Shinsuke Ohshima,
Noboru Hasegawa,
Masahiko Ishino,
Tetsuya Kawachi,
Hodaka Numasaki,
Teruki Teshima,
Hiroaki Nishimura
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Radiation Research
Vol. 174 • No. 4
October 2010
Vol. 174 • No. 4
October 2010