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26 October 2010 UVB Photoprotective Role of Mycosporines in Yeast: Photostability and Antioxidant Activity of Mycosporine-Glutaminol-Glucoside
Martín Moliné, Ernesto M. Arbeloa, María R. Flores, Diego Libkind, María Eugenia Farías, Sonia G. Bertolotti, María Sandra Churio, María Rosa van Broock
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Abstract

Several yeast species are able to synthesize and accumulate UV-radiation-absorbing mycosporine metabolites that are of unclear physiological function. In this work we analyzed the relationship between mycosporine-glutaminol glucoside (MGG) production, cell survival after UVB irradiation, and formation of cyclobutane pyrimidine dimers (CPDs). We also assessed the photostability and singlet oxygen quenching activity of MGG. A set of nine isolates of the basidiomycetous yeast Cryptococcus steppossus cultured in both dark and light conditions was used for the studies. Survival of the UVB-irradiated isolates and MGG concentration had a linear relationship when the concentration was over 2.5 mg g−1. CPD accumulation and MGG accumulation were inversely related. MGG in aqueous solution was photostable with a photodecomposition quantum yield of 1.16 × 10−5. MGG quenching of singlet oxygen was also observed, and the rate constant for the process in D2O was 5.9 × 107 M−1 s−1. Our results support the idea that MGG plays an important role as a UVB photoprotective metabolite in yeasts by protecting against direct damage on DNA and probably against indirect damage by singlet oxygen quenching.

Martín Moliné, Ernesto M. Arbeloa, María R. Flores, Diego Libkind, María Eugenia Farías, Sonia G. Bertolotti, María Sandra Churio, and María Rosa van Broock "UVB Photoprotective Role of Mycosporines in Yeast: Photostability and Antioxidant Activity of Mycosporine-Glutaminol-Glucoside," Radiation Research 175(1), 44-50, (26 October 2010). https://doi.org/10.1667/RR2245.1
Received: 15 April 2010; Accepted: 1 August 2010; Published: 26 October 2010
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