Covalently Linked Tandem Lesions in DNA

Helen B. Patrzyc; Jean B. Dawidzik; Edwin E. Budzinski; Harold G. Freund; John H. Wilton; Harold C. Box

doi:10.1667/RR2915.1

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Helen B. Patrzyc,¹ Jean B. Dawidzik,¹ Edwin E. Budzinski,¹ Harold G. Freund,¹ John H. Wilton,¹ Harold C. Box^1,*

¹Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York

^*Address for correspondence: Department of Cell Stress Biology, Roswell Park Cancer Institute, Carlton and Elm Sts., Buffalo, NY 14263; e-mail: harold.box@roswellpark.org.

Abstract

Reactive oxygen species (ROS) generate a type of DNA damage called tandem lesions, two adjacent nucleotides both modified. A subcategory of tandem lesions consists of adjacent nucleotides linked by a covalent bond. Covalently linked tandem lesions generate highly characteristic liquid chromotography-tandem mass spectrometry (LC-MS/MS) elution profiles. We have used this property to comprehensively survey X-irradiated DNA for covalently linked tandem lesions. A total of 15 tandem lesions were detected in DNA irradiated in deoxygenated aqueous solution, five tandem lesions were detected in DNA that was irradiated in oxygenated solution.

Citation Download Citation

Helen B. Patrzyc, Jean B. Dawidzik, Edwin E. Budzinski, Harold G. Freund, John H. Wilton, and Harold C. Box "Covalently Linked Tandem Lesions in DNA," Radiation Research 178(6), 538-542, (29 October 2012). https://doi.org/10.1667/RR2915.1

Received: 21 December 2011; Accepted: 1 July 2012; Published: 29 October 2012

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