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29 December 2017 The Sequence Preference of Gamma-Radiation-Induced Damage in End-Labeled DNA after Heat Treatment
Shweta D. Gautam, Megan E. Hardie, Vincent Murray
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In this work, we examined the DNA sequence preference of gamma-radiation-induced DNA damage in purified DNA sequences after heat treatment. DNA was fluorescently end-labeled and gamma-radiation-induced DNA cleavage was examined using capillary electrophoresis with laser-induced fluorescence detection. Our findings provide evidence that gamma-radiation-induced DNA damage to end-labeled DNA is nonrandom and has a sequence preference. The degree of cleavage was quantified at each nucleotide, and we observed that preferential cleavage occurred at C nucleotides with lesser cleavage at G nucleotides, while being very low at T nucleotides. The differences in percentage cleavage at individual nucleotides ranged up to sixfold. The DNA sequences surrounding the most intense radiation-induced DNA cleavage sites were examined and a consensus sequence 5′-AGGC*C (where C* is the cleavage site) was found. The highest intensity gamma-radiation-induced DNA cleavage sites were found at the dinucleotides, 5′-GG*, 5′-GC*, 5′-C*C and 5′-G*G and at the trinucleotides, 5′-GG*C, 5′-TC*A, 5′-GG*G and 5′-GC*C. These findings have implications for our understanding of ionizing radiation-induced DNA damage.

©2018 by Radiation Research Society.
Shweta D. Gautam, Megan E. Hardie, and Vincent Murray "The Sequence Preference of Gamma-Radiation-Induced Damage in End-Labeled DNA after Heat Treatment," Radiation Research 189(3), 238-250, (29 December 2017).
Received: 18 July 2017; Accepted: 1 November 2017; Published: 29 December 2017

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