Translator Disclaimer
6 April 2018 Analysis of Radiation-Induced Chromosomal Aberrations on a Cell-by-Cell Basis after Alpha-Particle Microbeam Irradiation: Experimental Data and Simulations
Antonella Testa, Francesca Ballarini, Ulrich Giesen, Octávia Monteiro Gil, Mario P. Carante, John Tello, Frank Langner, Hans Rabus, Valentina Palma, Massimo Pinto, Clarice Patrono
Author Affiliations +
Abstract

There is a continued need for further clarification of various aspects of radiation-induced chromosomal aberration, including its correlation with radiation track structure. As part of the EMRP joint research project, Biologically Weighted Quantities in Radiotherapy (BioQuaRT), we performed experimental and theoretical analyses on chromosomal aberrations in Chinese hamster ovary cells (CHO-K1) exposed to α particles with final energies of 5.5 and 17.8 MeV (absorbed doses: ∼2.3 Gy and ∼1.9 Gy, respectively), which were generated by the microbeam at the Physikalisch-Technische Bundesanstalt (PTB) in Braunschweig, Germany. In line with the differences in linear energy transfer (approximately 85 keV/μm for 5.5 MeV and 36 keV/μm for 17.8 MeV α particles), the 5.5 MeV α particles were more effective than the 17.8 MeV α particles, both in terms of the percentage of aberrant cells (57% vs. 33%) and aberration frequency. The yield of total aberrations increased by a factor of ∼2, although the increase in dicentrics plus centric rings was less pronounced than in acentric fragments. The experimental data were compared with Monte Carlo simulations based on the BIophysical ANalysis of Cell death and chromosomal Aberrations model (BIANCA). This comparison allowed interpretation of the results in terms of critical DNA damage [cluster lesions (CLs)]. More specifically, the higher aberration yields observed for the 5.5 MeV α particles were explained by taking into account that, although the nucleus was traversed by fewer particles (nominally, 11 vs. 25), each particle was much more effective (by a factor of ∼3) at inducing CLs. This led to an increased yield of CLs per cell (by a factor of ∼1.4), consistent with the increased yield of total aberrations observed in the experiments.

©2018 by Radiation Research Society.
Antonella Testa, Francesca Ballarini, Ulrich Giesen, Octávia Monteiro Gil, Mario P. Carante, John Tello, Frank Langner, Hans Rabus, Valentina Palma, Massimo Pinto, and Clarice Patrono "Analysis of Radiation-Induced Chromosomal Aberrations on a Cell-by-Cell Basis after Alpha-Particle Microbeam Irradiation: Experimental Data and Simulations," Radiation Research 189(6), 597-604, (6 April 2018). https://doi.org/10.1667/RR15005.1
Received: 5 December 2017; Accepted: 1 February 2018; Published: 6 April 2018
JOURNAL ARTICLE
8 PAGES


Share
SHARE
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top