Translator Disclaimer
24 August 2018 Computational Modeling and Clonogenic Assay for Radioenhancement of Gold Nanoparticles Using 3D Live Cell Images
Wonmo Sung, Yoon Jeong, Hyejin Kim, Hoibin Jeong, Clemens Grassberger, Seongmoon Jung, G-One Ahn, Il Han Kim, Jan Schuemann, Kangwon Lee, Sung-Joon Ye
Author Affiliations +
Abstract

Radioenhancement of gold nanoparticles (GNPs) has shown great potential for increasing the therapeutic efficiency of radiotherapy. Here we report on a computational model of radiation response, which was developed to predict the survival curves of breast cancer cells incubated with GNPs. The amount of GNP uptake was estimated using inductively coupled plasma-mass spectroscopy, and the three-dimensional (3D) intracellular distribution of GNPs was obtained using optical diffraction tomography. The developed computational model utilized the 3D live cell imaging and recent Monte Carlo techniques to calculate microscopic dose distributions within the cell. Clonogenic assays with and without GNPs were performed to estimate the radioenhancement for 150 kVp X rays in terms of cell survival fractions. Measured cell survival fractions were comparable with the computational model.

©2018 by Radiation Research Society.
Wonmo Sung, Yoon Jeong, Hyejin Kim, Hoibin Jeong, Clemens Grassberger, Seongmoon Jung, G-One Ahn, Il Han Kim, Jan Schuemann, Kangwon Lee, and Sung-Joon Ye "Computational Modeling and Clonogenic Assay for Radioenhancement of Gold Nanoparticles Using 3D Live Cell Images," Radiation Research 190(5), 558-564, (24 August 2018). https://doi.org/10.1667/RR15134.1
Received: 10 May 2018; Accepted: 9 August 2018; Published: 24 August 2018
JOURNAL ARTICLE
7 PAGES


Share
SHARE
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top