Herein we describe two new species of the Ischnocnema lactea species series. The new species were collected in Itatiaia highlands, located in Itatiaia National Park, southeastern Brazil. Ischnocnema melanopygia sp. nov. is characterized by dorsal stripes generally present; dark stripe on the cloacal area, tarsus and feet; calcar well developed; green yellowish and dark coloration on the inguinal region and posterior area of thigh in life. Ischnocnema concolor sp. nov. is characterized by absence of calcar; presence of well developed supernumerary tubercles on hands and feet; dorsum smooth, brown in preservative, without any observable pattern. These species are allocated in the Ischnocnema lactea species series and are compared to all other species of the group.