Anew species, belonging to the clade of Hypsiboas polytaenius, is described from the region of Veredas de Botumirim (17°07′S, 43°02′W, 659 m altitude), Municipality of Botumirim, State of Minas Gerais, Southeastern Brazil. The new species is characterized by its medium size (CRA 25.9–31.8 mm in males; 33.6 mm in female), head width about 27% of SVL, snout rounded in dorsal and lateral views, supra-cloacal crest and calcar absent, fingers with developed discs, similar to toe discs, webbing poorly developed between fingers, moderately developed between toes, and dorsal color pattern composed of four longitudinal pale brown stripes, separated by three narrow dark brown stripes, each pale brown stripe having dark brown longitudinal lines.