We studied diet composition, microhabitat use and daily activity patterns of an Andean population of Mabuya in a disturbed tropical habitat to evaluate if these features differed sexually, ontogenetically, seasonally, and among reproductive stages. Lizards ate mainly crickets plus grasshoppers, spiders and cockroaches; these prey were the most important in terms of volume and number. There were no significant differences in the number, type and abundance of prey, as well as in the length and volume of the consumed prey between males and females. There were significant differences between the diet of adults and juveniles. Lizards ate prey with similar volumes and lengths throughout the year independent of the rainfall regime. Also, there were no significant differences in prey volume and length with regard to the reproductive stages of females and males. The lack of variation in diet in this population suggests a constant availability of prey in the study area. When we compared diet composition of this population with that of other species of Mabuya, we found that diet tends to be similar among the different species and populations of Mabuya in South America. Adult lizards showed two activity peaks during the day, before and after midday; this pattern of daily activity was constant throughout the year. Preferred microhabitats used by this population are related to anthropogenic disturbance. The population of the present study seems to have quite wide ecological requirements (generalist diet, use of a wide spectrum of microhabitats and extended daily activity), which allows it to exploit disturbed habitats.