Corynorhinus rafinesquii (Rafinesque's Big-eared Bat) is uncommon and unevenly distributed throughout its range, and its populations are believed to be declining. The distribution in Florida, the southernmost extent of the range, is unclear, and recently published maps show records in only 16 counties scattered across the state. We compiled published and unpublished records of Rafinesque's Big-eared Bat in Florida and found 59 total records, including records from 13 counties where the species had not been previously reported. These new records expand the known distribution in Florida to 29 counties, including Miami-Dade County in extreme southeastern Florida. Our compilation of records clarifies the species' distribution in Florida, but several broad geographic areas still lack records despite the presence of forested wetland habitat potentially suitable for Rafinesque's Big-eared Bat. We recommend further survey efforts for Rafinesque's Big-eared Bat in 4 areas we identified where the species may be present.