The diversity, clinical manifestations, and impact on overall health of pathogenic fungi in Spilogale putorius (Eastern Spotted Skunk [ESS]) have been poorly investigated. Herein, we describe the first reported cases of fungal dermatitis in ESS from a population in southeastern Texas. In 2016, two of 3 ESS live-trapped in Harris County, TX, exhibited symptoms consistent with dermatophytosis. We isolated fungus from 1 of 2 cultures grown from scraped samples of infected ESS skin and morphologically identified it as Microsporum canis. Reviewing local wildlife rehabilitation records, we were able to confirm the prevalence of dermatophytosis within local mephitid populations. We examine the implications of the discovery of a zoonotic fungal pathogen in ESS in light of its importance in wildlife medicine as well as its ramifications for the health of domestic animals and humans.