We conducted baited camera-trap surveys in eastern Kentucky during October 2017–April 2018 to assess the efficacy of survey duration and attractant type in detecting Spilogale putorius (Eastern Spotted Skunk) and other Appalachian meso-mammals. We surveyed 16 sites across 10 counties over more than 1200 trap-nights. We observed meso-mammals of 9 species, including 3 records of Eastern Spotted Skunks. Our results indicated that a 2-week survey duration with sardine bait yielded meso-mammal detection rates, richness estimates, and species accumulation curves comparable to a 4-week survey duration with sardine bait; the addition of fatty acid scent tablets did not affect results. However, Eastern Spotted Skunks were not recorded until an average of 15 days after deployment, suggesting that species-specific trapping rates must be considered in the duration of camera-trap surveys.