The grapevine leaffolder, Desmia funeralis Hübner, is considered a secondary pest, but there have been economic infestations of this lepidopteran in the United States. During March-November 2016, natural parasitism and predation of this leaffolder in commercial vineyards, Vitis vinifera L., were recorded at Parras, Coahuila, Mexico. Samples were collected using direct, drop sheet-knockdown, and sweep net collection, to obtain larvae and pupae that were kept in laboratory until adults hatched from the leaffolder or parasitoids. Parasitoids detected were Nemorilla pyste Walker 1849 (Diptera: Tachinidae), Bracon cushmani Fabricius 1805 (Hymenoptera: Braconidae) and Brachymeria ovata Say 1824 (Hymenoptera: Chalcididae). Total leaffolders parasitized were 83%, with 42.9% parasitized by N. pyste, 17.6% by B. cushmani, and 4.2% by B. ovata. Regression analysis of parasitism data indicated that there was a direct, positive relationship between D. funeralis abundance and percent parasitism. The spider Enoplognatha ovata (Clerck, 1757) was a predator on larvae and adults of D. funeralis.