Seven species of macrochelid mites new to the Italian fauna are recorded here: Holostaspella ornata (Berlese, 1904), H. pulchella Mašán, 2003, Macrholaspis recki (Bregetova & Koroleva, 1960), Macrocheles decoloratus(C. L. Koch, 1839), M. punctoscutatus Evans & Browning, 1956, M. rotundiscutus Bregetova & Koroleva, 1960 and Neopodocinum meridionalis (Sellnick, 1931). A description of the male of Holostaspella pulchella is given here, highlighting the existence of specialised ventral structures with unknown function previously unknown in the Macrochelidae. Records also are provided for other two species previously recorded from Italy without specific locality data and missing from the Italian fauna checklist, Macrocheles mammifer Berlese, 1918 and Nothrholaspis montanus Willmann, 1951. The first documented records for Nothrholaspis tardus (C.L. Koch, 1841) (sensu Evans & Browning, 1956) from Italy definitively confirm its synonymy with Macrocheles tridentinus (G. & R. Canestrini, 1882), a species originally described from Italy. Updated and detailed data about geographical distribution and ecology are provided for each species.