In the course of a two-month Franco-Chilean expedition in January and February 2006, we found nine species of oribatid mites from nine genera and eight families. Two new species of oribatid mites, Pseudantarcticola aquatica sp. nov. and Multioppia chilensis sp. nov., are described from benthic samples from Chile. Pseudantarcticola georgiae (Wallwork, 1970) comb. nov. is included in the genus Pseudantarcticola from Antarcticola. Identification keys to known species of Pseudantarcticola and to Chilean species of Multioppiaare presented.