Four new species of mites of the genus Hispiniphis are described, all among those upon which the original description of the genus was based, associated with beetles of different species of the hispine genus Cephaloleia. A key is given to the described species of Hispiniphis and Makarovaia, the known two genera whose adults and immatures are found under the elytra of rolled-leaf hispine beetles. Aspects of ontogenetic acceleration and duplication of some idiosomatic setae are discussed. Evidence of larviparity, along with problematical differences between small dimensions of the adult female genital area and a relatively large larva, are considered. An evolutionary trend towards smaller body sizes among these mites is discussed. Further evidence of host preferences by these mites is presented, along with indications that there may be a vast diversity of these mites associated with hispine beetles in the Neotropical Region.