The mite Oligonychus punicae has potential for development in Eucalyptus spp. in clonal minigardens. However, there is no information on its biological and reproductive parameters on eucalyptus species. The objective of this study is to evaluate the biological and reproductive performance of O. punicae on different species of eucalyptus cultivated in a clonal minigarden. Development time, survival, longevity, fecundity and life table parameters of O. punicae were determined on six species of eucalyptus (Eucalyptus urophylla, Eucalyptus brassiana, Eucalyptus grandis, Eucalyptus tereticorinis, Eucalyptus pellita and Corymbia citriodora). All eggs of O. punicae originated females and completed the cycle in all hosts. Differences were observed in development, longevity, pre-oviposition, oviposition and post-oviposition, and fecundity of females of O. punicae when different eucalyptus species were used as hosts for O. punicae. The best performance of O. punicae was observed on E. tereticornis, while the worst performance was observed on C. citriodora, on which the net reproductive rates (R₀) ranged from 5.09 to 44.85, the intrinsic rate of population increase (r_m) ranged from 0.09 to 0.19, the finite rate of population increase (λ) ranged from 1.10 to 1.21, and the mean generation time (T) ranged from 0.09 to 0.19. These results suggest that E. tereticornis is an excellent host for O. punicae.