Ixodes lividus Koch, 1844 (Ixodida: Ixodidae) is a specialised endophilic tick of the sand martin Riparia riparia whose distributional range matches that of its host in the Paleartic region. However, the presence of this tick species has not been described in Mediterranean countries where the sand martin also breeds. In this study, we investigated I. lividus infestation in 1,081 Riparia riparia individuals from two breeding colonies in central Portugal. In total, three I. lividus were collected from three sand martins (infestation rate of 0.28%). A molecular survey on these ticks did not detect the presence of any Borrelia burgdorferi s.l., Rickttesia spp. nor Babesia spp. This report expands to the south and west the known distributional range of I. lividus in Europe.