The family Winterschmidtiidae is subdivided into four subfamilies; representatives of two are associated with insects and those of other two commonly inhabit decaying materials, fungi, plant leaves, vertebrate nests and stored food. Species of this family are frequently collected during surveys of arboreal acarofauna in Brazil, but in most cases, they are identified up to the generic level. The present work reports winterschmidtiid species collected in agricultural environments in Brazil: a new species, Oulenziella longisetasp.nov., and four previously known species, Acalvolia squamata (Oudemans), Oulenzia arboricola (Oudemans), Oulenziella bakeri (Hughes) and Czenspinskia transversostriata (Oudemans). Among these, A. squamata, O. arboricola and O. bakeri are new records for Brazil.