Species of the mite genus Protzia Piersig, 1896 are diverse and sometimes highly abundant freshwater mites. Here, we provide COI barcodes for five species (Protzia rugosa, P. rotunda, P. halberti, P. cabardinica, P. longiacetabulata) and use them for species delimitation analyses accompanied with morphological comparisons. As a result, we resurrected the species P. cabardinica (Sokolow, 1940). Based on morphology only, we describe three new species, P. kyrgyzicasp. nov. and P. tienshanensissp. nov., from Kyrgyzstan and P. iranicasp. nov. from southern Iran. Furthermore, we describe the male of P. longiacetabulata from Turkey, which was not known previously.