Molecular methods have brought new insight to the study of mating patterns. Extrapair fertilizations (EPF) have proven to be widespread among bird species irrespective of social relationships. Ecological factors, such as breeding density and synchrony, have been suggested as contributors to variation in EPF rates. Absence of a male during the female fertile period may also increase the probability of EPFs. In this study, we examined experimentally whether breeding density and male absence before egg-laying influence the EPF rate in Pied Flycatchers (Ficedula hypoleuca). DNA fingerprinting revealed that 4.4% (10/225) of nestlings were the result of EPFs and 7.0% (3/43) of broods included at least one offspring sired by a male other than that attending the nest. We did not find any evidence of higher EPF rate with higher breeding density as stated by the density hypothesis. Contrary to expectation, EPFs were more frequent, though not quite statistically significantly, at low breeding density: all three EPF nests were found in low-breeding-density areas. There was no evidence that EPF rate was affected by a 4 h male removal. In all cases of EPF, an old female was mated with a yearling male, which suggests that older females mated with browner yearling males may have adjusted their initial mate choice through EPCs to acquire indirect genetic benefits. Pied Flycatcher females may be constrained to accept a mate of lower than preferred quality, especially at low breeding density, due to the cost of searching for alternative males.