Lichen diversity along the Mississippi River in the Minneapolis-St. Paul urban area was studied in 2008 by sampling 37 localities along the 110-kilometer river basin, resulting in 76 species, which are thought to represent 80% of the complete flora. A significant decrease in lichen diversity toward the urban part of the river basin, as well as the presence of only one cyanolichen suggests that urban factors, e.g. air pollutants and habitat destruction may be affecting lichen diversity. In addition, lichen diversity has significantly decreased from that of 112 years ago, when a study by Fink reported 113 species, and a higher number of genera represented by a single species.