Four new species of Desmopachria Babington are described from Bolivia: D. amyae Miller, new species, D. challeti Miller, new species, D. signatoides Miller, new species, and D. volatidisca Miller, new species. The female genitalia of members of the genus are described and illustrated in detail for the first time. A discussion of the taxonomy and characters historically used for the classification of subgenera and species groups is presented. It is concluded that recognition of subgenera in the genus, despite the large size of the group and existence of good characters for grouping some species, is not desirable since some groups are weakly supported by character evidence and/or are probably paraphyletic. Use of all previous subgenus names is rejected. The species are organized into informal groups, most of which correspond with historically used subgenera. Several species are left ungrouped.