The Neotropical spider genus Paradossenus is revised and currently comprises a total of 14 species. P. andinus (Simon 1898), P. protentus (Karsch 1879) and P. venezuelanus (Simon 1898) are new junior synonyms of P. longipes (Taczanowski 1874), the type species of the genus. Five known species, P. longipes, P. caricoi Sierwald 1993, P. pulcher Sierwald 1993, P. corumba Brescovit & Raizer 2000, and P. minimus (Mello-Leitão 1940), are redescribed and illustrated. New species: P. isthmus, P. benicito, P. amazonensis, P. acanthocymbium, P. tocantins and P. pozo are described from both male and female. The new species P. sabana is described only from the male while P. junin is described only from the female. The subfamily Trechaleinae is erected, diagnosed, and an illustrated key to all the included genera is presented.