Development of novel microsatellite markers for the spider genus Loxosceles (Sicariidae) using next-generation sequencing

Enric Planas; Laia Bernaus; Carles Ribera

doi:10.1636/B13-88.1

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Enric Planas, Laia Bernaus, Carles Ribera

Abstract

We report the step-by-step process of developing de novo microsatellite (SSR) loci in two Loxosceles spider species. We used reads obtained with next-generation sequencing (Roche 454) to select hundreds of potentially-amplifiable SSRs. After testing amplification and cross-amplification, we characterized 18 SSRs, 11 of which were polymorphic in Loxosceles rufescens (Dufour 1820) and seven of which were polymorphic in L. sp. Fuerteventura - Lanzarote. This method is a relatively fast and economic procedure for the development of fast-evolving nuclear markers in spiders.

The American Arachnological Society

Citation Download Citation

Enric Planas, Laia Bernaus, and Carles Ribera "Development of novel microsatellite markers for the spider genus Loxosceles (Sicariidae) using next-generation sequencing," The Journal of Arachnology 42(3), 315-317, (1 November 2014). https://doi.org/10.1636/B13-88.1

Received: 19 December 2013; Published: 1 November 2014

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