Detailed descriptions of sperm induction are rare in the literature. Anelosimus vierae Agnarsson, 2012 (Theridiidae) is a Uruguayan subsocial spider that lives in communal nests. This paper describes the sperm induction of this species under laboratory conditions. We collected spiders in Montevideo, Uruguay, from different trees and placed them in thirty male and female pairs in Petri dishes to observe the occurrence of sperm induction. We witnessed twenty-two males performing sperm induction after copulation. Sperm web characteristics and description of the male's behavior during sperm induction are described.