Based on specimens from several museum collections and recently sampled spiders during a field excursion to Mexico in 2014, the 11 species of Tegenaria s. l. endemic to the United States of America and Mexico are revised. Morphological characters and mitochondrial DNA sequences (CO1, NADH1, 16S) serve as the basis for proposed new combinations and new species. Tegenaria chiricahuae Roth, 1968 remains the only endemic Tegenaria species in the Western Hemisphere. All other specific names (T. blanda Gertsch, 1971, T. caverna Gertsch, 1971, T. decora Gertsch, 1971, T. flexuosa F.O. Pickard-Cambridge, 1902, T. florea Brignoli, 1974, T. gertschi Roth, 1968, T. mexicana Roth, 1968, T. rothi Gertsch, 1971, T. selva Roth, 1968, and T. tlaxcala Roth, 1968) are transferred to the genus Eratigena Bolzern, Burckhardt & Hänggi, 2013. Six new species are described: E. edmundoi, E. fernandoi, E. guanato, E. queretaro, E. xilitla, and E. yarini. In addition, females of E. flexuosa, and E. gertschi, and the male of E. florea are described for the first time. A phylogeny based on maximum likelihood analysis of combined mtDNA sequences, an identification key and images of all diagnosed species are provided.