We sequenced small subunit ribosomal DNA (rDNA) PCR-fragments of sizes 2.3 kb and 2.9 kb isolated from a culture of the red alga, Porphyra spiralis var. spiralis. Phylogenetic analysis of the 2.3-kb fragment showed that it encoded the sequence of a contaminant filose amoeba. The Nuclearia-like amoeba (named strain N-Por) was identified with scanning electron microscopy. Its rDNA sequence was positioned with strong bootstrap support within a diverse protist assemblage that includes filose amoebae, chlorarachniophytes, cercomonads, and Plasmodiophora brassicae. The rDNA of N-Por contained a group I intron at the conserved 943 position that remarkably, had a U at the 3′-terminus rather than the universally conserved G.
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1 November 2000
The SSU rDNA Coding Region of a Filose Amoeba Contains a Group I Intron Lacking the Universally Conserved G at the 3′-Terminus
DEBASHISH BHATTACHARYA,
MARIANA C. OLIVEIRA
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The Journal of Eukaryotic Microbiology
Vol. 47 • No. 6
November 2000
Vol. 47 • No. 6
November 2000
Cercozoa
Group I intron
phylogeny
ribosomal DNA
splicing