Encephalitozoon hellem is a microsporidian species responsible for opportunistic infections in AIDS patients. Use of a novel chitinase-based method allowed unsheared chromosomal DNA to be recovered from eleven E. hellem isolates derived from three geographic regions. All isolates were typed by 18S rDNA sequencing, which showed that they belonged to internal transcribed spacer type 1. After ethidium bromide staining, pulsed-field gel electrophoresis (PFGE) analysis discriminated two new karyotypes comprising 7 and 8 chromosomal bands respectively, ranging in size from 205- to 272-kb pairs. Genomic size was estimated to be 2.39 Mb. Our data indicate PFGE is useful for typing E. hellem and confirms genetic diversity among E. hellem genotypes.
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1 July 2001
Genetic Diversity in the Microsporidian Encephalitozoon hellem Demonstrated by Pulsed-Field Gel Electrophoresis
SEBASTIEN DELARBRE,
SIMONETTA GATTI,
MASSIMO SCAGLIA,
MICHEL DRANCOURT
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The Journal of Eukaryotic Microbiology
Vol. 48 • No. 4
July 2001
Vol. 48 • No. 4
July 2001
genome size
karyotypes
microsporidia