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1 November 2002 Azadirachtin Disrupts Formation of Organised Microtubule Arrays during Microgametogenesis of Plasmodium berghei
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Transmission of malaria parasites from vertebrate blood to the mosquito vector depends critically on the differentiation of the gametocytes into gametes. This occurs in response to environmental stimuli encountered by the parasite in the mosquito bloodmeal. Male gametogenesis involves three rounds of DNA replication and endomitosis, and the assembly de novo of 8 motile axonemes. Azadirachtin, a plant limnoid and insecticide with an unkown mode of action, specifically inhibits the release of motile gametes from activated microgametocytes but does not inhibit growth and replication of asexual blood stages. We have combined confocal laser scanning microscopy and transmission electron microscopy to examine the effect of azadirachtin on the complex reorganisation of the microtubule cytoskeleton during gametogenesis in Plasmodium berghei. Neither the replication of the genome nor the ability of tubulin monomers to assemble into microtubules upon gametocyte activation were prevented by azadirachtin. However, the drug interfered with the formation of mitotic spindles and with the assembly of microtubules into typical axonemes. Our observations suggest that azadarachtin specifically disrupts the patterning of microtubules into more complex structures, such as mitotic spindles and axonemes.

OLIVER BILLKER, MICHAEL K. SHAW, IAN W. JONES, STEVEN V. LEY, A. JENNIFER MORDUE (LUNTZ), and ROBERT E. SINDEN "Azadirachtin Disrupts Formation of Organised Microtubule Arrays during Microgametogenesis of Plasmodium berghei," The Journal of Eukaryotic Microbiology 49(6), 489-497, (1 November 2002).
Received: 31 March 2002; Accepted: 18 September 2002; Published: 1 November 2002

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