Identification of the Inositol Isomers Present in Tetrahymena

MICHAEL C. KERSTING; MICHELE BOYETTE; JOSEPH H. MASSEY; PHILLIP E. RYALS

doi:10.1111/j.1550-7408.2003.tb00111.x

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1 May 2003 Identification of the Inositol Isomers Present in Tetrahymena

MICHAEL C. KERSTING, MICHELE BOYETTE, JOSEPH H. MASSEY, PHILLIP E. RYALS

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The J. of Eukaryotic Microbiology, 50(3):164-168 (2003). https://doi.org/10.1111/j.1550-7408.2003.tb00111.x

Abstract

The inositol isomer composition of phosphoinositides, polyphosphoinositols, phosphatidylinositol-linked glycans, and glycosyl phosphatidylinositol-anchored proteins of logarithmic phase Tetrahymena vorax was determined by GC-MS analysis of trimethylsilylimadazole derivatives. The most abundant inositol found was the myo-isomer; however, appreciable percentages of scyllo-inositol were present in the free inositol pool, phosphatidylinositol-linked glycan fraction, and glycosyl phosphatidylinositol-anchored protein fraction. Trace quantities of chiro- and neo-inositols also were present.

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MICHAEL C. KERSTING, MICHELE BOYETTE, JOSEPH H. MASSEY, and PHILLIP E. RYALS "Identification of the Inositol Isomers Present in Tetrahymena," The Journal of Eukaryotic Microbiology 50(3), 164-168, (1 May 2003). https://doi.org/10.1111/j.1550-7408.2003.tb00111.x

Received: 9 October 2002; Accepted: 28 January 2003; Published: 1 May 2003

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